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U.S. National Center For Environmental Research

Digestive Solubilization of Sediment-Sorbed Contaminants: A Comparison of In Vitro and In Vivo Processes
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The goal of the proposed work is to confirm and expand upon early work, and contrast in vitro digestive fluid solubilization with in vivo digestive processes, bioavailability and bioaccumulation. The in vitro technique allows the researchers to answer mechanistic questions by manipulating sediment or digestive fluid characteristics in ways that would not be possible in vivo, but first there is a need to establish that in vitro solubilization provides meaningful information in the context of the whole animal's accumulation of sediment-sorbed contaminants. Included within this objective are contrasts between the novel digestive fluid solubilization approach and more traditional means of measuring bioavailability such as absorption (assimilation) efficiency, uptake clearance rates and steady state body burdens. The researchers then intend to expand the research to examine its generality to multiple species and varied substrate types.
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